Medicago truncatula-Ralstonia solanacearum

Jiffy pellets grown Madicago truncatula plants are cut at their bottom third and soaked in a bacterial suspension at 10*8 cfu/ml, wilting is observed around 5 days after inoculation and the whole bioassay is completed around 15-20 days after inoculation. In vitro grown Medicago truncatula are inoculated directly on the exposed roots with 200µl/plant of a bacterail suspension at 10*8 cfu/ml, wilting can be observed after 10 days, the assays is considered finished at 21 days post inoculation. the maximum of bacterial colonization is usually obersed at around 10 days post-inoculation.